FIGURE 1.

SH-SY5Y cells were seeded as 1 × 10⁵ cells/ml of DMEM containing 10% FBS or 1% FBS or 1% BSA and used for experiments after overnight incubation. Cells were incubated with the absence or presence of different concentrations of ROT (0, 0.5, 1, 2.5, 5, and 10 μM) for 24 h and assessed for trypan blue assay (A) or TH, PINK1, Parkin, α-syn and β-actin by Western blotting (B). In addition, cells were fractionated into 1% Triton X-100 soluble and insoluble fractions and analyzed for α-syn by Western blotting (C). Each picture is a representative of three independent experiments. Data are mean ± SEM of three independent experiments and analyzed by one-way of variance (ANOVA) followed by Tukey’s post hoc test. Statistical significance: _acompared with 10% FBS control; _bcompared with 1% FBS control; _ccompared with 1% BSA control; ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001.