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. 2019 Sep 24;10:834. doi: 10.3389/fgene.2019.00834

Figure 5.

Figure 5

Complexity of spliced transcripts. (A) The splice sites of UL34-35 transcript were confirmed by dRNA sequencing. The splicing event leads to frameshifting of the ul34 ORF in UL34-35-SP2 and UL34-35-L-SP2 and in the deletion of the translational initiation site of the ul34 ORF in UL34-35- SP1 UL34-35-L2-SP1. (B) The splicing complexity of RL2 and novel non-coding and potentially coding transcripts overlapping RL1 and the 5’ UTR of RL2. Alternative splicing in RL2-SP1 produces a frameshift in the second half of the ORF, while alternative splicing in RL2-SP2 and in RL2 results in premature stop codons. Two novel 5’-UTR length isoforms of the RL2 transcripts are shown, one of which may contain a truncated form of the RL1 ORF. This truncation is caused by a splicing event. A novel isoform of the RL1 with longer 5’ and 3’ UTRs (designated as RS1-L1-AT) was discovered. Another novel putative protein coding transcript is the RS2-ALT, which is co-terminal with RS1-L1-AT.