Skip to main content
. 2019 Sep 24;13:431. doi: 10.3389/fncel.2019.00431

FIGURE 2.

FIGURE 2

Calcium influx via reversal of NCX is not required to induce retrograde hyper-excitability post-axotomy. (A) Representative images of presynaptic terminals labeled with FM5-95 (FM puncta) before and after field stimulation within the somatodendritic compartment of microfluidic chambers. Color look up table ‘Fire’. Scale bars, 10 μm. (B,C) FM unloading curves at 48 h following application of KBR (B) or TTX (C) to axonal compartment for 1 h during axotomy. Uninjured control: n = 782 puncta; axotomy: n = 1037 puncta; Axot. KBR: n = 1012 puncta; Axot. TTX: 1415 puncta. (D) FM unloading curves at 48 h following 10 min application of veratridine to axonal compartment without injury. Control (DMSO): n = 711 puncta; veratridine: n = 1273 puncta. Two-way ANOVA, Bonferroni post hoc test. Inset in B–D shows FM decay time constant (τ) for puncta with τ < 360s (control: n = 654; Axot. Veh.: n = 977; Axot. KBR: n = 945 puncta; Axot. TTX: 1321 puncta). (D) control, n = 634; veratridine, n = 1177. Unpaired two-tailed t-test. 5–6 chambers for each condition over 3 independent experiments. ∗∗p < 0.001. Error bars, s.e.m.