FIGURE 4.

ER calcium channel activation at the site of injury is required for retrograde inhibitory terminal loss post-axotomy. (A) Representatrive mCherry labeled dendritic segments immunostained for the inhibitory presynaptic marker, vGAT, at 48 h following application of vehicle or local activity blockade solution (ABS) with or without Dantrolene and Xestospongin C to axons for 1 h during axotomy. Arrows in the merge image indicate the localization of vGAT puncta (cyan) on mcherry labeled dendrites (red). Scale bar, 5 μm. (B,C) Number of vGAT puncta per neuron area (uninjured control, axotomy or axotomy + ABS and axotomy + ABS + Dan + Xes) at 15-16 DIV. n = 6–8 neurons; 3 chambers per condition over 2 independent experiments. Error bars, SEM. ^∗p < 0.05.