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. 2019 Sep 24;10:2273. doi: 10.3389/fimmu.2019.02273

Figure 1.

Figure 1

Expression of miR-21 in human macrophages and murine DCs. (A) Small RNAseq results showing the enrichment of microRNAs and snoRNAs in LdWT infection compared to LdCen−/− infected human macrophages differentiated in vitro from elutriated monocytes. (B) Human macrophages, infected with LdWT or LdCen−/− showing elevated expression of miR-3651, miR21, and miR-3679 in LdWT compared to LdCen−/− infection. (C) Expression of miR-21 5p in murine DCs infected with LdWT or LdCen−/− parasites at 3, 6, and 12 h post infection. (D) Expression of miR-21 3p in murine DCs infected with LdWT or LdCen−/− parasites at 3, 6, and 12 h post infection. The fold increase in expression is compared to that observed in naïve DCs. (E) qRT-PCR assay showing the parasite burden in the murine DCs infected with LdWT or LdCen−/− parasites at the indicated time points. (F) Expression of IL-12p35 mRNA in the murine DCs either in presence or absence of anti-miR-21 5p oligonucleotides delivered through exosomes is measured by qRT-PCR. (G) Expression of IL-12 in the culture supernatants of murine DCs infected with LdWT or LdCen−/− parasites 12 h post-infection in presence or absence of anti-miR-21 oligonucleotides by ELISA. (H) Expression of TNF protein in the culture supernatants of macrophages infected with LdWT or LdCen−/− parasites measured by ELISA is shown. Statistical significance between miR-21 or IL-12 expression in LdWT and LdCen−/− infections at various time points post infection is shown (t-test; *p < 0.05, **p < 0.01, ***p < 0.001).