Table 2. Median Staining Intensity Scores of Renal Striatin and Caveolin-1 Localization.
Striatin | Caveolin-1 | |||||
---|---|---|---|---|---|---|
Sham | Aldo | Ep.+Aldo | Sham | Aldo | Ep.+Aldo | |
Cortex | ||||||
GL | 1 | 3 | 2 | 1 | 4 | 3 |
PCT | 1 | 2 | 2 | 0 | 1 | 1 |
DCT | 1 | 2 | 2 | 0 | 1 | 1 |
CCD | 2 | 3 | 2 | 0 | 1 | 1 |
Pcap | 1 | 3 | 3 | 3 | 4 | 4 |
Outer medulla | ||||||
TALH | 2 | 2 | 3 | 1 | 2 | 1 |
MCD | 2 | 2 | 4 | 1 | 2 | 1 |
VR | 3 | 4 | 4 | 4 | 4 | 4 |
tLH | 3 | 3 | 4 | 0 | 2 | 1 |
Inner medulla | ||||||
MCD | 3 | 4 | 4 | 1 | 1 | 1 |
VR | 3 | 4 | 4 | 2 | 4 | 4 |
tLH | 2 | 3 | 4 | 2 | 1 | 2 |
Staining intensity: 0, negative, no reactivity; 1, trace, faint or pale brown staining with less membrane reactivity; 2, weak, light brown staining with incomplete membrane reactivity; 3, moderate, shaded of brown staining of intermediate darkness with usually almost complete membrane reactivity; 4, strong, dark brown to black staining with a usually complete membrane pattern, producing a thick outline of the cell [17,18,19].
Aldo, aldosterone; Ep., eplerenone (n=6/group); GL, glomerulus; PCT, proximal convoluted tubule; DCT, distal convoluted tubule; CCD, cortical collecting duct; Pcap, peritubular capillary; TALH, thick ascending limb of the loop of Henle; MCD, medullary collecting duct; VR, vasa recta; tLH, thin limb of the loop of Henle.