Fig. 2.

Genetic interaction between kibra, rhea, and human Tau in the eye of Drosophila. (A) Table presenting the homology of WWC1 and TLN2 with their Drosophila orthologs. (B and C) Image and size quantification of fly eyes expressing the 2N4R Tau isoform (GMR>Tau) in loss-of-function (in blue) and gain-of-function ([GOF], in green) kibra conditions (scale bar 0.1 mm). The GMR>images correspond to the same kibra conditions without Tau expression. Numbers above the x axis in the graphs indicate the number of eyes that were quantified. Knockdown (overexpression) of kibra rescued partially (enhanced) Tau toxicity in the eye (C. right graph). This was likely an additive effect of the modulation of kibra with Tau as knockdown (overexpression) of kibra alone increased (decreased) the size of the eyes (C. left graph). However, 1 haploinsufficient condition, kibra^2/+, partially rescued Tau toxicity (C. right graph) without affecting the eye on its own (C. left graph). (D and E) Image and size quantification of fly eyes expressing the 2N4R Tau isoform (GMR>Tau) in loss-of-function (in blue) rhea conditions (scale bar 0.1 mm). Expression of Tau in the haploinsufficient rhea^1/+ background resulted in bigger eyes (E. right graph), whereas haploinsufficient rhea^1/+ flies have similar eye size than control (E. left graph), suggesting a genetic interaction between Tau and rhea. Abbreviation: WWC1, WW and C2 domain containing 1, aka KIBRA.