Figure 2.

Identification of the SLC27A1 transcriptional start site. (A) Nested PCR was used to generate SLC27A1 5′ RACE products that were subjected to agarose gel electrophoretic separation, with arrows corresponding to the amplicons. (B) The initiation sites for the two SLC27A1 mRNA variants identified via 5′ RACE are shown along with a partial genomic structure for this gene, and the initiation codon ATG are indicated with arrows. White boxes identify constitutive exons, while the black box highlights the alternatively spliced region. Introns are indicated by solid lines and dashed lines indicating potential splicing variations.