miR-152 is more abundant in the slow-twitch myofiber-enriched muscles. (A) Metachromatic adenosine triphosphatase (ATPase) staining of the longissimus dorsi (LD) muscles from large white (LW) and Rongchang (RC) pigs (n = 5, five pigs). Type I fibers stain light gray. Original magnification, ×100. Bars: 100 μm. (B) Proportion of the slow-twitch fiber was analyzed in the LD muscles from LW and RC pigs based on the ATPase staining. (C) miR-152 expression in the LD muscles from both LW and RC pigs was determined by RT-PCR (n = 5, five pigs). (D) Tissue distribution of miR-152 in adult LW pigs (n = 5, five pigs). (E) Expression of miR-152 in different types of skeletal muscle in adult LW pigs (n = 5, five pigs). Results are exhibited as mean ± S.E.M. ** p < 0.01 and *** p < 0.001 when contrasted with the control.