Table 5.
Validation of RNA sequencing results of samples in pigs feeding one time (M1) or twice daily (M2) by real-time quantitative PCR.
| Gene 3 | RNA Sequencing 1 (M1 vs. M2) | Real-time PCR 2 (M1 vs. M2) | ||
|---|---|---|---|---|
| p Value | Fold Change | p Value | Fold Change | |
| APOA1 | 0.004 | 1.981 | 0.033 | 1.821 |
| APOA4 | <0.001 | 2.879 | 0.028 | 2.287 |
| APOC3 | <0.001 | 2.620 | 0.039 | 2.383 |
| NPC1L1 | 0.041 | 1.545 | 0.045 | 1.954 |
| ACAA1 | 0.013 | 1.549 | 0.042 | 1.319 |
| FABP1 | 0.001 | 2.320 | 0.042 | 2.476 |
| PCK1 | 0.020 | 1.733 | 0.044 | 1.612 |
1 Results based on RNA sequencing of six samples from two groups. 2 Results based on six individual samples from each group. 3 APOA1: Apolipoprotein A1; APOA4: Apolipoprotein A4; APOC3: Apolipoprotein C3; NPC1L1: Niemann-Pick C1-Like 1; ACAA1: Acetyl-CoA acyltransferase 1; FABP1: fatty acid binding protein 1; PCK1: Phosphoenolpyruvate carboxykinase 1.