Figure 3.

Expression of human apoptosis-related genes in HT-29 cells treated with GnRH-[⁴Ser] conjugates for 24 and 48 h. Effects of I-[⁴Ser,⁶D-Lys(Dau)] (A), II-[⁴Ser,⁶D-Lys(Dau)] (B), III-[⁴Ser,⁸Lys(Dau)] (C) on gene expression were analyzed by a human apoptosis gene PCR array (RealTime ready custom panel). The colors of the heatmap show significant fold changes in gene expression compared to control. Fold changes ≥ 2 and p < 0.05 were considered as significant. A hashed zone means invalid PCR results. TP53: tumor protein p53 data; BAD: BCL2-associated agonist of cell death; BAX: BCL2-associated X protein; BAK1: BCL2-antagonist/killer 1; BCL2: B-cell CLL/lymphoma 2; TNF: tumor necrosis factor (TNF)-alpha, tumor necrosis factor ligand superfamily member 2; TNFRSF8: tumor necrosis factor receptor superfamily, member 8; TRAF7: TNF receptor-associated factor 7; FASL: Fas ligand, TNF superfamily member 6 (TNFSF6); FAS: TNF receptor superfamily member 6 (TNFRSF6); FADD: Fas (TNFRSF6)-associated via death domain; CASP7: caspase 7; CASP3: caspase 3; CASP9: caspase 9; CASP8: caspase 8; HMGB1: high-mobility group box 1; NFKB1: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; RELA: v-rel reticuloendotheliosis viral oncogene homolog A; AKT1: v-akt murine thymoma viral oncogene homolog 1; PTEN: phosphatase and tensin homolog; STAT1: signal transducer and activator of transcription 1; SOCS2: suppressor of cytokine signaling 2; HSP90B1: heat shock protein 90 kDa beta (Grp94) member 1.