Table 3.
The apoptosis-related genes investigated and the primers used in this study.
| Gene | Gene Name | Forward and Reverse Primers | |
|---|---|---|---|
| Intrinsic Apoptotic Pathway | TP53 | Tumor protein p53 data | AGGCCTTGGAACTCAAGGAT, CCCTTTTTGGACTTCAGGTG |
| BAD | BCL2-associated agonist of cell death | CTACGGTGGGAGAGGAAGC, TGTTACGTAGTCAAGGCACAGC |
|
| BAX | BCL2-associated X protein | GGACGTGGGCATTTTTCTTA, GTTTATTACCCCCTCAAGACCA |
|
| BAK1 | BCL2-antagonist/killer 1 | AGACCTGAAAAATGGCTTCG, CGGAAAACCTCCTCTGTGTC |
|
| BCL2 | B-cell CLL/lymphoma 2 | GCACCTGCACACCTGGAT, AGCCAGGAGAAATCAAACAGAG |
|
| Extrinsic Apoptotic Pathway | TNF | TNF-alpha, tumor necrosis factor ligand superfamily member 2 | CGGTGCTTGTTCCTCAGC, GCCAGAGGGCTGATTAGAGA |
| TNFRSF8 | Tumor necrosis factor receptor superfamily, member 8 | GCTGTCAGGAGGTGCTGTTAC, GTAGGCCTCTGTGGGCACT |
|
| TRAF7 | TNF receptor-associated factor 7 | ATGTCTCTGCGCTCCACATT, AGCTGACAGCACAGCTTCAC |
|
| FASL | Fas ligand, TNF superfamily, member 6 | CAGTTCTTCCCTGTCCAACC, GTGGTGGTGGCCTCCTTT |
|
| FAS | TNF receptor superfamily, member 6 | TGAGGAAGACTGTTACTACAGTTGAGA, GCAGTCCCTAGCTTTCCTTTC |
|
| FADD | Fas (TNFRSF6)-associated via death domain | AGGTAGCCCAGCACTGTGAA, AGGTGGTCTGTGGCTCACTC |
|
| Effector Apoptotic Proteins | CASP7 | Caspase 7 | CCCGCAAAGCAACGTCTA, CCCCTGCTCTTCAATACAGC |
| CASP3 | Caspase 3 | CTGGTTTTCGGTGGGTGT, CAGTGTTCTCCATGGATACCTTTATT |
|
| CASP9 | Caspase 9 | CCCAAGCTCTTTTTCATCCA, AGTGGAGGCCACCTCAAAC |
|
| CASP8 | Caspase 8 | GAAAGGGTGGAGCGGATT, GATTTCTGCTGAAGTCCATCTTTT |
|
| HMGB1 | High-mobility group box 1 | GAGTGAGGAGGCTGCGTCT, TGCCCATGTTTAGTTATTTTTCC |
|
| Cytokine/Growth-Factor Signaling Pathway | NFKB1 | Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 | CTGGCAGCTCTTCTCAAAGC, TCCAGGTCATAGAGAGGCTCA |
| RELA | v-rel reticuloendotheliosis viral oncogene homolog A | ACCGCTGCATCCACAGTT, GGATGCGCTGACTGATAGC |
|
| AKT1 | v-akt murine thymoma viral oncogene homolog 1 | GCAGCACGTGTACGAGAAGA, GGTGTCAGTCTCCGACGTG |
|
| PTEN | Phosphatase and tensin homolog | GCTACCTGTTAAAGAATCATCTGGA, CTGGCAGACCACAAACTGAG |
|
| STAT1 | Signal transducer and activator of transcription 1 | GGATTGAAAGCATCCTAGAACTCA, GATGAAGCCCATGATGCAC |
|
| Anti-Apoptotic Proteins | SOCS2 | Suppressor of cytokine signaling 2 | AGGCCTCACTGCAATTTGAT, TGCAAAATATAAAATGCCCAAG |
| HSP90B1 | Heat shock protein 90 kDa beta (Grp94), member 1 | CTGGAAATGAGGAACTAACAGTCA, TCTTCTCTGGTCATTCCTACACC |