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. 2019 Sep 17;20(18):4587. doi: 10.3390/ijms20184587

Figure 2.

Figure 2

VAC promotes eNOS phosphorylation in high glucose (HG)-exposed human microvascular endothelial cell-1 (HMEC-1) cells. The HMEC-1 cells pre-incubated with vaccarin (5 μM) for 12 h or eNOS inhibitor L-NAME (100 μM) for 1 h prior to 30 mM HG exposure. (A) Nitric oxide (NO) generation was determined with ELISA. n = 5 for each group; (B, C) Representative blots showing protein expressions of p-eNOS in HMEC-1 cells. Bar groups showing the quantification of p-eNOS/t-eNOS, n = 3 for each group; (D) NO generation. n = 5 for each group. Values are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. NG, normal control group; HC, hyperosmotic control group; HG, high glucose group; VAC, vaccarin; L-NAME, NG-Nitro-L-arginine Methyl Ester.