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. 2019 Sep 17;20(18):4587. doi: 10.3390/ijms20184587

Figure 3.

Figure 3

The role of miRNA-34a in VAC-promoting NO production. HMEC-1 cells were incubated with vaccarin (5 μM) for 12 h, or miRNA-34a inhibitor or mimic for 6 h and then stimulated with 35 mM HG for 48 h. (A) Effects of vaccarin (5 μM) on the miRNA-34a expression in response to HG. Effects of inhibition of miRNA-34a on HG-stimulated miRNA-34a expression (B), NO generation (C), eNOS phosphorylation (D and E). Effects of miRNA-34a mimic on HG-stimulated miRNA-34a expression (F), NO generation (G), eNOS phosphorylation (H and I). n = 3 for each group in D, E, H and I; n = 5 for other groups. Values are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. NC, negative control; INH, inhibitor.