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Compound 1 inhibited glutamate-induced apoptotic HT22 cell death. (A) HT22 cells were treated with 5 mM glutamate in the presence of 1 for 12 h and stained with Hoechst33342. Fluorescent images were obtained using a fluorescence microscope (40×). (B) HT22 cells were treated with 5 mM glutamate for 12 h in the presence of 1 and stained with Alexa Fluor 488-conjugated annexin V and propidium iodide. Fluorescent images were obtained using a Tali Image-Based Cytometer.
