Skip to main content
. 2019 Sep 13;11(9):1363. doi: 10.3390/cancers11091363

Figure 1.

Figure 1

KRIBB11 attenuated mevalonate and cholesterol biosynthesis-related gene expression in hepatocellular carcinoma (HCC) cells. (A,B) SK-HEP-1, PLC/PRF5, and Huh7 cells were pretreated with KRIBB11 (2 μM) for 1 h and further treated with simvastatin (10 μM) for 24 h. The mRNA levels were measured using quantitative real-time polymerase chain reaction (qRT-PCR). The values represent the mean ± SD of two independent experiments performed in triplicate. (C) SK-HEP-1 cells were cultured in lipoprotein-depleted fetal bovine serum (DL-FBS) for 6 h, and then cells were further incubated with KRIBB11 (2 μM) for 24 h. The mRNA levels were measured using qRT-PCR. The values represent the mean ± SD of two independent experiments performed in triplicate. (D) SK-HEP-1 cells were incubated under simvastatin (10 μM) treatment or DL-FBS for 1 h or 6 h, respectively, and then KRIBB11 was further incubated for 24 h. The expression of proteins was measured by western blotting. (E) H1299, HCT116, and A2058 cells were cultured in lipoprotein-depleted fetal bovine serum (DL-FBS) for 6 h, and then cells were further incubated with KRIBB11 (2 μM) for 24 h. The mRNA levels were measured using qRT-PCR. The values represent the mean ± SD of two independent experiments performed in triplicate. * p < 0.05 and ** p < 0.01.