Figure 3.
Effects of Delphinol® on in vitro osteoclastogenesis. (A) Cell proliferation of BMM was examined by BrdU incorporation assay. Bone marrow cells (3 × 105 cells per cm2) were cultured in α-MEM containing 10% FBS supplemented with 4000 U of Leukoprol for 2 days and incubated for 4 h in the presence of BrdU. BrdU incorporation was detected based on a colorimetric ELISA assay kit. (B) Experimental protocol for osteoclastogenesis. (C) Representative photographs of tartrate-resistant acid phosphatase (TRAP) staining in osteoclasts (OC) cultures. Scale bars = 50 μm. (D) Anti-osteoclastogenic activity of maqui berry extract (MBE) was examined by the intensity of TRAP staining at 540 nm on a spectrophotometer. (E) qPCR analyses of Nfatc1 and CtsK mRNA expression. The level of mRNA expression was normalized with that of Gapdh. Values are expressed as mean ± SD (n = 3–4). *p < 0.05, **p < 0.01, ***p < 0.001.