Figure 6.
Role of the HRE and ARE in Prdx6 gene expression. (A) Schematic illustration of the Prdx6 promoter containing ARE and two HRE sites (a black box for Nrf-2 binding site, white boxes for HIF-1α binding sites). Deletion of the ARE or two HREs were generated by site-directed mutagenesis of the full-length promoter (pGL3-mPx) in the pGL3-Basic vector, namely pGL3-mPxΔARE and pGL3-mPxΔHRE. (B) Each reporter vector and the β-galactosidase expression plasmid were cotransfected with the empty pcDNA3 vector, the Nrf-2, or HIF-1α expression plasmid into HEI-OC1 cells. After 48 h, cells were incubated with 200 μM CoCl2 for 6 h, and the luciferase activity was determined. Luciferase activities were normalized to that of β-galactosidase. Data are presented as the means ± SDs of three independent experiments. * p < 0.05, compared with the untreated control (NC), # p < 0.05, pGL3-mPx group versus pGL3-mPxΔARE or pGL3-mPxΔHRE group.