Figure 2.

Isolation of IgE+ B lymphocytes from PBMC of donors with different type of allergy. (A) Gating strategy for detection of IgE+ B lymphocyte. B lymphocyte subset were gated as CD3-CD14-CD16- (FSC area/CD3, CD14, CD16), single cells (FSC area/FSC height), CD19+, and then detection of IgE+ B cells was based on surface IgE expression. (B) Example of different percentage of IgE+ B cells in donor samples with different types of allergy. The highest percentage of IgE+ cells were detected in a donor sample with seasonal allergy shortly after the pollen season (6th June) and they disappeared a month later (4th July). As a control, we used blood samples from healthy donors, as well as donors with year-round allergies.