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. 2019 Aug 22;8(9):328. doi: 10.3390/antiox8090328

Figure 2.

Figure 2

Effects of high glucose on cellular and mitochondrial reactive oxygen species (ROS). Upper panels: Micro images of CellROX-stained cells showing cellular ROS formation (green florescence) in HuREC treated for 48 h with high glucose (HG; 25 mM d-glucose), normal glucose (NG; 5 mM d-glucose), and the osmotic control L-glucose (LG; 25 mM l-glucose). Lower panels: Micro images of MitoSOX stained cells showing mitochondrial ROS formation (red florescence) in HuREC treated for 48 h with high glucose (HG; 25 mM d-glucose), normal glucose (NG; 5 mM d-glucose), and the osmotic control L-glucose (LG; 25 mM l-glucose). DAPI was used for nuclear counterstain (blue fluorescence).