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. 2019 Aug 22;8(9):952. doi: 10.3390/cells8090952

Figure 1.

Figure 1

IFNγ ELISPOT response in the spleen following intranasal infection with F. tularensis. Mice were infected with a sub-lethal dose (102 cfu, 0.1 LD50) of LVS strain, or with a lethal dose of 105 cfu LVS or 102 Schu strain (100 LD50). Animals were sacrificed and splenocytes were stimulated in situ in an ELISPOT assay with UV-inactivated LVS (UViLVS, 5E7 cfu equivalent/mL) or left untreated (no antigen). Images of 106 cells/well of splenocytes from naïve (A) or sub-lethal LVS-infected mice (day 3 post infection) (B) are presented. (C) Quantification of IFNγ spots represent groups of 3 mice, collected in the indicated time-points following sub-lethal LVS infection, and individually assayed as described above. (D) Three mice were collected 3 days following lethal intranasal infection with LVS (105 cfu) or Schu strain (102 cfu), and assayed as described above. Data are representative of three (LVS infections) and two (SchuS4 infections) independently performed experiments. ** p < 0.01.