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Exosomal marker expression in liver cells. Cells were plated on 10 cm dishes and either cultured in growth medium without further treatment (NC), or subjected to viral infection with a control virus (Luc). Thereafter, exosomes were prepared from the supernatants and marker expression was analyzed by Western blot using antibodies directed against Alg2-interacting protein (Alix), CD81 and Caveolin-1 (Cav-1).
