Figure 6.

Identification of full-length Endoglin in primary mesenchymal liver cells. (A) Cell protein extracts, supernatant (pre), wash solution (post), and final exosome preparations from primary rat HSC untreated (NC), infected with adenoviral constructs expressing luciferase (Luc) or full-length endoglin (FL-Eng) were analysed by Western blot for expression of indicated proteins. (B) Primary rat HSC were stimulated or not with TGF-β1 (1 ng/mL) for 24 h and exosomes were prepared. The corresponding proteins were analysed by Western blot for the expression of exosomal marker proteins and FL-Eng. (C) Cell lysates and corresponding exosomal proteins of three independent experiments of primary rat pMF were analysed by Western blot for exosomal markers and TGF-β-receptors. For displaying equal protein loading, the membrane was reprobed with a β-actin specific antibody. Note that in contrast to other liver cell lines and primary cells, rat pMF express significant amounts of Tβ-RIII and Tβ-RI in exosomes.