PGE1-Containing Protocols Generate Mature (Leukemia-Derived) Dendritic Cells Directly from Leukemic Whole Blood

. 2019 Sep 17;20(18):4590. doi: 10.3390/ijms20184590

(A)

	MLC^{WB-DC Kits}	MLC^WB	Uncultured Cells	p-Values
	% of Cells in the Corresponding Subgroup			MLC^{WB-DC Kits} vs. MLC^WB	MLC^{WB-DC Kits} vs. Uncultured Cells	MLC^WB vs. Uncultured Cells
T Cell Subtypes
CD3⁺CD4⁺/CD3⁺	44.7 ± 16.1	57.0 ± 18.6	63.6 ± 21.4	<0.08*	<0.02**	<0.44
CD3⁺CD8⁺/CD3⁺	55.3 ± 16.1	43.0 ± 18.6	36.4 ± 21.4	<0.08*	<0.02**	<0.44
Proliferating T Cells
CD3⁺CD71⁺/CD3⁺	22.8 ± 18.3	17.8 ± 9.2	1.1 ± 0.6	<0.3	<0.000001***	<0.00001***
CD3⁺CD69⁺/CD3⁺	23.0 ± 16.7	18.4 ± 15.5	3.1 ± 4.4	<0.4	<0.00001***	<0.009***
Non-Naïve or Naïve T cells
CD3⁺CD45RO⁺/CD3⁺	73.5 ± 15.9	67.0 ± 16.9	48.8 ± 15.6	<0.3	<0.0003***	<0.02**
CD3⁺CD8⁺CD45RO⁺/CD3⁺	34.6 ± 13.4	26.4 ± 14.6	16.8 ± 12.4	<0.13	<0.001***	<0.14
CD3⁺CD4⁺CD45RO⁺/CD3⁺	38.9 ± 17.3	40.7 ± 16.1	32.0 ± 12.5		<0.19	<0.17
CD3⁺CD45RO⁻ ^{(including subsets)}	26.2 ± 15.6	32.7 ± 16.9	51.1 ± 15.5	<0.3	<0.0003***	<0.02**
Effector (Memory) T Cells
CD3⁺CCR7⁻CD45RO⁺/CD3⁺	62.8 ± 18.9	55.5 ± 21.8	36.8 ± 16.6	<0.23	<0.0005***	<0.04**
CD3⁺ CCR7⁺CD45RO⁺/CD3⁺	10.3 ± 14.0	11.5 ± 15.5	11.9 ± 16.7	<0.84	<0.79	<0.95