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. 2019 Sep 12;9(9):481. doi: 10.3390/biom9090481

Figure 3.

Figure 3

Digestion of membrane chondroitin sulfate and heparin and inhibition of glycoaminoglycan synthesis decrease membrane fixation of LL-37 and cell migration. (a) Migration of MDA-MB-231, MDA-MB-435s and MCF7 cell lines induced by LL-37. Cells were previously incubated or not with different enzymes digesting glycoaminoglycans (Hep: heparinases I/III, treatment 5mUI/mL 1 h, ABC: chondroitinase ABC, treatment 1 UI/mL 1 h, Xylo: 4-Methylumbelliferyl-β-d-xyloside 0.5 mM) or with an inhibitor of sulfatation (SC—sodium chlorate 30 mM) (N = 4). (b) Immunofluorescence labeling with anti-LL-37 on MDA-MB-231 cells previously treated or not as in (a). Magnification ×400. Data are normalized to LL-37. Statistics: *** p < 0.001, ** p < 0.01, * p < 0.05, relative to LL-37 and relative to control without LL-37, respectively.