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. 2019 Sep 11;20(18):4477. doi: 10.3390/ijms20184477

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of the butyric acid. The fermented media from glycerol (G) fermentation by S. epidermidis bacteria (B) analyzed by HPLC. (a) Chromatograph of only media (M), media with glycerol (M + G), media with bacteria (M + B), and media with bacteria plus glycerol (M + B + G). The x-axis is retention time in minutes, and the y-axis is the milli-absorbance unit at 210 nm. (b) The concentrations of BA (butyric acid) was quantified from the height of butyric acid standard (STD) peaks. Data shown represent the mean ± SE of experiment performed in triplicate. *** p < 0.0001 (two-tailed t-test).