. 2019 Aug 23;11(9):1992. doi: 10.3390/nu11091992

Table 2.

Summary of flow cytometry technique characteristics in selected studies

Study ID	Sample	Treatment for IEL Isolation	Gating Strategy	TCRγδ+ Definition	CD3⁻ Definition
Camarero 2000 [15]	Duodenum or proximal jejunum	RPMI 10% FBS, 1 mM DTT, 1mM EDTAShacker, 60 min, RT	IEL: CD45⁺, lowSSC	CD45⁺TCRγδ⁺	CD45⁺CD3⁻CD7⁺
Calleja 2011 [14]	3 biopsies distal duodenum	RPMI, 1 mM DTT, 1 mM EDTA60 min	IEL: CD45⁺, lowSSC, CD103⁺	CD45⁺TCRγδ⁺ CD103⁺	CD45⁺CD3⁻CD103⁺
Fernández-Bañares 2014 [24]	1 biopsy 2nd part of duodenum	HBSS, 1mM DTT, 1 mM EDTAShacker, 90 min, RT	IEL: CD45⁺, lowSSC	CD45⁺TCRγδ⁺	CD45⁺CD3⁻
Valle 2017 [25]	2 biopsies 2nd part of duodenum	RPMI 10% FBS, 1 mM DTT, 1mM EDTAShacker, 60 min, RT	IEL: CD45⁺, lowSSC	CD45⁺TCRγδ⁺	CD45⁺CD3⁻CD103⁺
Saborido 2018 [26]	1 biopsy	RPMI 10% FBS, 1 mM DTT, 1mM EDTA60 min	IEL: CD45⁺, lowSSC, CD103⁺	CD45⁺TCRγδ⁺ CD103⁺	CD45⁺CD3⁻CD103⁺
Nijeboer 2019 [27]	6 biopsies	PBS, 1 mM DTT, 1 mM EDTA60 min	IEL: CD45⁺, lowSSC	CD45⁺TCR γδ ⁺	CD45⁺surfaceCD3⁻intrCD3⁺ CD7⁺

DTT: ditiotreitol; EDTA: ethylenediaminetetraacetic acid; RPMI: Roswell Park Memorial Institute medium; FBS: phosphate-buffered saline; HBSS: Hanks′ balanced salt solution; SSC: side scatter; IEL: intraepithelial lymphocytes.