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. 2019 Sep 7;11(9):2144. doi: 10.3390/nu11092144

Figure 5.

Figure 5

TP53 expression influences the iron-dependent regulation of IRP1 and IRP2 protein expression. Representative Western blots of IRP1 and IRP2 protein expression in TP53 null H1299 cells (H1299) or following the induction of the indicated TP53 subtype and treatment with 40 µM hemin (A) or 50 µM DFO (D) for 24 h. Relative expression levels were quantitated following normalization to anti-gamma-tubulin (TUBG1) as the loading control (B,C,E,F). * Denotes statistical difference from respective controls, p < 0.05.