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Impact of WT and mutant TP53 expression on Fe-S cluster biogenesis. Representative Western blot analysis of ferredoxin reductase (FDXR) expression in TP53 null cells (H1299) and following tetracycline induction of indicated TP53 expression types (A). FDXR protein quantitation following normalization to TUBG1 (B). Cytosolic (C) and mitochondrial (D) aconitase activity were also measured in the same cell types. * Denotes statistical difference, p < 0.05.
