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. 2019 May 21;40(37):3081–3094. doi: 10.1093/eurheartj/ehz308

Figure 5.

Figure 5

Cytoskeleton in cardiomyocytes derived from induced pluripotent stem cells obtained from the proband (BrS1) and his unaffected brother (Ctl1). (A) Left: representative immunostainings of filamentous actin (F-actin; stained with phalloidin) and troponin I and merged acquisitions with nucleus staining with DAPI in Ctl1 and BrS1 cardiomyocytes derived from induced pluripotent stem cells (top panels) and corresponding fluorescence distribution measured at the level of the yellow dotted lines (fluorescence-distance relationships; bottom panels). Scale bars: 20 µm. Right panel: percentage of Ctl1 (n = 10) and BrS1 (n = 18) cardiomyocytes derived from induced pluripotent stem cells with striated (in white) and cortical (in black) F-actin staining. Statistical test: Fisher’s exact test. (B) Left: representative three-dimensional acquisition illustrations of a Ctl1 and a BrS1 cardiomyocytes derived from induced pluripotent stem cell after immunostaining of F-actin and Troponin I (left panels). Width: 107.12 µm; height: 12 µm; and depth: 107.12 µm. Right panel: box plots of thickness and cell circularity (cell width/cell length ratio) in Ctl1 (n = 10) and BrS1 (n = 12) cardiomyocytes derived from induced pluripotent stem cells Mann–Whitney test.