Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 22;8(9):956. doi: 10.3390/cells8090956

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Inhibition of GSK3β activity promotes disinhibition of RGC neurite outgrowth in the presence of inhibitory Nogo-P4 peptide. (A) Immunocytochemistry showing disinhibited neurite outgrowth from βIII-tubulin⁺ RGC in the presence of Nogo-P4 peptide after treatment with siGSK3β whilst control cultures treated with sNBA, Lipofectamine 2000, or siEGFP were unable to achieve disinhibited neurite outgrowth. CNTF-treated cultures were used as positive controls. (B) Quantification of the proportion of RGC bearing neurites and (C) the mean longest RGC neurite after siRNA treatment in the presence of Nogo-P4 peptide, showing significant disinhibition of neurite outgrowth after treatment with siGSK3β. (Scale bar in A = 20 μm; ** = p < 0.01). n = 2 wells/treatment, 3 independent repeats (total n = 6 wells/treatment).