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. 2019 Sep 11;8(9):1064. doi: 10.3390/cells8091064

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The volcano plot analysis of proteomic from supernatant of RAW264.7 cells after sequential lipopolysaccharide (LPS) stimulation (LPS tolerance; LPS/LPS) compared with control (N/N) (see method) demonstrated down- and up-regulated proteins (A) and the Venn diagram indicated the different number of proteins from LPS tolerance (LPS/LPS) group versus the control (B). Additionally, pathway analysis clusters (DAVID) of up- and down-regulated proteins (C,D) and the enriched pathway of the proteins in LPS/LPS compared with the control (E) were demonstrated.