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. 2019 Sep 12;9(9):482. doi: 10.3390/biom9090482

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The autophagic flux was blocked by EBV and restored by quercetin. (a) Sequestosome1/p62 (SQSTM1/p62) expression level was investigated following EBV infection of B cells treated or not with quercetin at 24 and 72 h post-infection. (b) Microtubule-associated protein 1 light chain 3-I/II (LC3-I/II) as evaluated by western blot analysis in EBV-infected B cells, treated or not with quercetin, in the presence or in the absence of Bafilomycin 1 (Baf), at 24 and 72 h post-infection. Densitometric analysis was performed using Image J software and the ratio of SQSTM1/p62 or LC3-II vs. b-actin was calculated. Histograms represent the mean ± SD of three independent experiments.