Figure 4.

Quercetin reduces reactive oxidative species (ROS) but does not affect BRLF1 expression in EBV-infected B lymphocytes. (a) Time-dependent increase of intracellular ROS evaluated by 2′,7′-dichlorofluorescein diacetate (DC-FDA) staining and FACS analysis. The mean of fluorescence intensity is reported on the Y-axis (b). (c) BRLF1 and EBNA1 expression levels as evaluated by western blot analysis following EBV infection of B cells, with or without treatment with quercetin. Densitometric analysis was performed using Image J software, and the ratio of BRLF1 vs. b-actin was calculated. Histograms represent the mean ± SD of three independent experiments.