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. 2019 Aug 21;8(9):327. doi: 10.3390/antiox8090327

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Roles of ROS on the genistein-induced G2/M arrest and apoptotic cell death. T24 cells were cultured in medium containing 160 μM genistein for 48 h or treated with 10 mM NAC for 1 h and then stimulated with genistein for 48 h. (A,B,D) The effect of NAC on the genistein-induced cell cycle arrest and apoptosis were investigated using flow cytometry analysis. (C) The nuclear morphology was observed using DAPI staining. (E) The effect of NAC on the genistein-induced cytotoxicity was evaluated by an MTT assay (*** p < 0.0001 compared to control; ^### p < 0.0001 compared to genistein-treated cells).