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. 2019 Aug 30;11(9):1276. doi: 10.3390/cancers11091276

Figure 4.

Figure 4

PC3-derived EVs activate caspase-1 via ERK1/2. PNT2 cells were treated with 100 µg/mL of PC3-derived EVs (PC3-EVs) for 24 h (A) in the presence or in the absence of 10 µg/mL LPS + 5 mM ATP (LPS + ATP) and the expression of phosphorylated ERK1/2 was analyzed by Western blotting. PNT2 cells were pretreated for 1 h with 10 µM U0126 and then treated for 24 h with 100 µg/mL PC3-EVs. (B) caspase-1 full length and mature caspase-1-p20 expression and (C) pro-IL-1β full length and mature IL-1β-p20 expression were evaluated in total extract analyzed by Western blotting. β-actin was used as a loading control. The images are representative of one out of n = 3 separate experiments. Numbers below the images represent the ratio between the respective protein and β-actin band intensity.