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. 2019 Sep 17;8(9):1096. doi: 10.3390/cells8091096

Figure 4.

Figure 4

Analysis of protein expression in undifferentiated and differentiated MO3.13 cells. (A) Western blot analyses of MBP (polyclonal), MOG, CNPase, MBP (monoclonal), TUBB3, PLP1 and PON2 in (+) human motor cortex, (1) undifferentiated MO3.13 cells, (2) differentiated MO3.13 cells following incubation in N2.1 medium, (3) N2.2 medium or (4) T3 medium (normalized to GAPDH expression). Western blot analyses were performed for two independent experiments (n = 2). (B) Example images of immunocytochemistry for CNPase and TUBB3 in undifferentiated MO3.13 cells and MO3.13 cells differentiated with N2.1 medium or N2.2 medium. Scale bar = 50 µm.