Figure 6.

Immunocytochemical analysis of neuronal differentiation in co-cultures of HOG or MO3.13 cells with differentiated neuronal SH-SY5Y cells. (A) Representative images of for expression of mature neuronal markers TUBB3 and tyrosine hydroxylase (TH) or dopamine beta-hydroxylase (DBH) in differentiated SH-SY5Y monocultures. (B) Representative images of Jagged1 and L1CAM surface expression and TUBB3 signals in SH-SY5Y monoculture, and SH-SY5Y + HOG and SH-SY5Y + MO3.13 co-cultures. Scale bar = 50 µm. (C) Quantifications of neurite caliber in low density neurite networks of differentiated SH-SY5Y cells (n = 2).