Table 1.

Differentiation media used in HOG and MO3.13 cell culture experiments. AA: antibiotic-antimycotic. db-cAMP: N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate. IBMX: 3-isobutyl-1-methylxanthine. PMA: phorbol 12-myristate 13-acetate.

Differentiation Medium	Components
N2.1 medium	Eagle’s Minimum Essential Medium (EMEM) 1% antibiotic-antimycotic (AA), 50 μg/mL apo-transferrin, 16 μg/mL putrescine, 0.5 μg/mL human insulin, 30 nM sodium selenite, 30 nM triiodothyronine (T3), 500 uM 3-isobutyl-1-methylxanthine (IBMX), 500 uM dibutyryl cAMP (db-cAMP)
N2.2 medium	EMEM 1% AA, 50 μg/mL apo-transferrin, 16 μg/mL putrescine, 0.5 μg/mL human insulin, 30 nM sodium selenite, 30 nM T3, 10 ng/mL D-biotin, 50 nM hydrocortisone, 4 μM progesterone
T3 medium	EMEM 1% AA, 30 nM T3
PMA medium	Gibco Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12(1:1)) 1% AA, 100 nM phorbol 12-myristate 13-acetate (PMA)