Table 2.
Bacterial strains and plasmids.
| Strain or Plasmid | Description | Source/Reference |
|---|---|---|
| Escherichia coli JM109 | endA1, recA1, gyrA96, thi, hsdR17 (rk−, mk+), relA1, supE44, Δ( lac-proAB), [F′ traD36, proAB, laqIqZΔM15] | CICIM-CU 1 |
| Bacillus licheniformis 9945a | Wild-type strain | CICIM-CU |
| Bacillus licheniformis GM2 | Bacillus licheniformis 9945a derivative, deficient in ΔamyL, ΔaprE | GY. Shi, this lab |
| pHY300-PLK | E.coli/Bacillus shuttle vector, ApR/TetR | TaKaRa |
| pBLxyl | pHY300-PLK derivative with the xylose regulon from B. licheniformis 9945a | This work |
| pBLSY | pHY300-PLK derivative with the xylose regulon from B. licheniformis 9945a, the signal peptide from the levansucrase-encoding gene of B. subtilis 168 (sacBss), and the maltogenic amylase (yvdF)-encoding gene and terminator sequence from B. licheniformis DSM13 | This work |
| pBSSY | pHY300-PLK derivative with the xylose regulon from B. subtilis 168, the signal peptide from the levansucrase encoding gene of B. subtilis 168 (sacBss), and the maltogenic amylase (yvdF)-encoding gene and terminator sequence from B. licheniformis DSM13 | GY. Shi, this lab |
1 CICIM-CU, Culture and Information Center of Industrial Microorganisms of China Universities; ApR, ampicillin resistance; TetR, tetracycline resistance.