Table 7.
Effect of feeding regime on microbial loads a of milk and cheese samples.
| Samples | Media b | |||||||
|---|---|---|---|---|---|---|---|---|
| PCA-SkM 7 °C | PCA-SkM 30 °C | MRS 30 °C | MRS 44 °C | M17 30 °C | M17 44 °C | KAA | VRBGA | |
| MDWB0 | 6.21 ± 0.70 | 6.21 ± 0.80 | 6.01 ± 0.89 | 2.95 ± 0.73 | 6.15 ± 0.98 | 4.63 ± 0.91 | 5.18 ± 0.38 | 4.62 ± 0.78 |
| MDWB10 | 6.18 ± 0.30 | 6.70 ± 0.37 | 5.34 ± 0.82 | 3.09 ± 0.64 | 6.89 ± 0.70 | 4.84 ± 0.94 | 4.68 ± 0.44 | 4.69 ± 0.42 |
| MDWB20 | 6.12 ± 0.33 | 6.08 ± 0.88 | 5.32 ± 0.74 | 3.02 ± 0.53 | 6.32 ± 0.73 | 4.55 ± 0.92 | 4.98 ± 0.65 | 4.60 ± 0.94 |
| P value | 0.973 | 0.569 | 0.537 | 0.965 | 0.541 | 0.925 | 0.512 | 0.987 |
| CDWB0 | 8.81 ± 0.63 | 8.95 ± 0.63 | 7.11 ± 0.63 | 7.21 ± 0.94 | 8.81 ± 0.30 | 8.35 ± 0.69 | 7.97 ± 0.69 | 5.78 ± 0.52 |
| CDWB10 | 8.79 ± 0.24 | 9.00 ± 0.57 | 7.37 ± 0.87 | 7.01 ± 0.81 | 8.85 ± 0.48 | 8.08 ± 0.47 | 7.33 ± 0.45 | 5.96 ± 0.54 |
| CDWB20 | 9.01 ± 0.26 | 9.09 ± 0.28 | 7.42 ± 0.71 | 7.10 ± 0.73 | 8.84 ± 0.50 | 8.18 ± 0.35 | 7.49 ± 0.90 | 5.59 ± 0.74 |
| P value | 0.783 | 0.945 | 0.864 | 0.958 | 0.993 | 0.820 | 0.546 | 0.767 |
a Units are log CFU/mL for milk samples and log CFU/g for cheese samples. Results indicate mean values ± standard deviation (SD) of four plate counts (carried out in duplicate for two independent productions). Abbreviations: MDWB0, control milk, 0% of durum wheat bran, MDWB10, experimental milk, 10% of durum wheat bran, MDWB20, experimental milk, 20% of durum wheat bran, CDWB0, control cheese, 0% of durum wheat bran, DWB, CDWB10, experimental cheese, 10% of durum wheat bran, CDWB20, experimental cheese, 20% of durum wheat bran. b PCA-SkM 7 °C, plate count agar added with skimmed milk incubated at 7 °C for total psychrotrophic microorganisms, PCA-SkM 30 °C, plate count agar added with skimmed milk incubated at 30 °C for total mesophilic microorganisms, MRS 30 °C, de Man-Rogosa-Sharpe agar for mesophilic rod LAB, MRS 44 °C, de Man-Rogosa-Sharpe for thermophilic rod LAB, M17 30 °C, medium 17 agar incubated at 30 °C for mesophilic coccus LAB, M17 44 °C, medium 17 agar incubated at 44 °C for thermophilic coccus LAB, KAA, kanamycin aesculin azide agar for enterococci, VRBGA, violet red bile glucose agar for Enterobacteriaceae.