Effects of the FF diet on iron parameters and of dietary iron on hepatic lipid accumulation. (A,B) Serum ferritin at 3–4 and 5–6 months (n = 3–5 per group). (C,D) Hepatic iron content at 3–4 and 5–6 months (n = 3–6 per group). (E,F) Hepcidin mRNA (Hamp) normalized to 18s rRNA at 3–4 and 5–6 months (n = 3–5 per group). (G,H) Bone morphogenetic protein 6 (Bmp6) mRNA at 3–4 and 5–6 months (n = 3–5 per group). (I,J) Liver triglyceride content (TG) at 3–4 and 5–6 months (n = 3–10 per group). Shown are means ± SE. p values were calculated by two-way ANOVA with post hoc Tukey’s multiple comparisons test. ns: not significant, i.e., p ≥ 0.05; * 0.01 ≤ p ≤ 0.05; ** 0.001 ≤ p ≤ 0.01; *** 0.0001 ≤ p ≤ 0.001; **** p < 0.0001.