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. 2019 Aug 28;11(9):1266. doi: 10.3390/cancers11091266

Figure 1.

Figure 1

Characterization of kifunensine-treated Mel-JuSo cells. (A) Protocol of ApoEV and ApoEV-HM production and isolation procedure. (B) Mel-JuSo cells were cultured with kifunensine for 72 h. Dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) binding was assessed using DC-SIGN-Fc and measured by flow cytometry. (C) Cell viability was measured by flow cytometry after staining with Annexin V and fixable viability dye (FVD). Representative of n = 3. (D) Mel-JuSo cells were treated with bortezomib to induce apoptosis. After 24, 48, and 72 h Annexin V (as a measure of apoptosis) and the cell viability (FVD) was measured by flow cytometry. Representative plots of n = 3. (E) After 72 h of apoptosis induction, cell viability ranged between approximately 5–25%. Data shown as mean ± SD of three individual experiments.