Figure 5.

Neurotrophin and inflammatory cytokine expression in the hippocampus of cold-stressed mice. (A) Brain-derived neurotrophic factor (BDNF), interleukin (IL)-1β, and β-actin levels were evaluated by western blotting and quantified by densitometric analysis. (B,C) Quantification of expression levels based on BDNF/β-actin (B) and IL-1β/β-actin (C) ratios. Differences between room temperature male (RTM) and cold exposure male (CEM), room temperature female (RTF) and cold exposure female (CEF), and CEM and CEF groups were evaluated by two-way analysis of variance. Results are expressed as the mean ± SD (n = 6) of four independent experiments. N.S.: not significant; * p < 0.05, *** p < 0.001.