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. 2019 Aug 30;9(9):426. doi: 10.3390/biom9090426

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Apoptosis in the dentate gyrus (DG) of cold-stressed mice. (A) Transferase dUTP Nick End Labeling (TUNEL)-positive cells (green) counterstained with 4’,6-diamidino-2-phenylindole in the DG. Scale bar = 50 μm. (B) Quantification of TUNEL-positive cells. Differences between room temperature male (RTM) and cold exposure male (CEM), room temperature female (RTF) and cold exposure female (CEF), and CEM and CEF groups were evaluated by two-way analysis of variance. Results are expressed as the mean ± SD (n = 6) of four independent experiments. N.S.: not significant; *** p < 0.001.