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. 2019 Aug 30;9(9):426. doi: 10.3390/biom9090426

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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mRNA expression of inflammatory cytokines and effects of cortisol (CORT) on the viability of BV2 cells. (A) Interleukin (IL)-1β mRNA level in BV2 cells following treatment with indicated concentrations of CORT (0–400 μM) for 3 h was evaluated by quantitative real-time (qRT)-PCR; cells treated with lipopolysaccharides (LPS) (1 μg/mL) served as a positive control. Differences among control, CORT, and LPS groups were evaluated by one-way analysis of variance. Results are expressed as the mean ± SD (n = 3) of three independent experiments. N.S.: not significant; *** p < 0.001. (B,C) BV2 cells were treated with different concentrations of CORT (0–500 μM) for 3 h and viability was assessed with the cell counting kit (CCK)-8 assay, and cells treated with LPS served as a positive control. Differences among control, CORT, and LPS groups were evaluated by one-way analysis of variance. Results are expressed as the mean ± SD (n = 3) of three independent experiment. N.S.: not significant; ** p < 0.01; *** p < 0.001.