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. 2019 Sep 5;11(9):2115. doi: 10.3390/nu11092115

Figure 1.

Figure 1

Growth curve of E. coli in continuous culture. E. coli K12 bacteria were cultured at 37 °C in Mueller-Hinton (MH) medium. Every 12 h, bacteria were supplemented with a new MH medium to imitate 2 daily meals (black arrow). After 48 h of culture (white arrow), ClpB mRNA and protein levels were analyzed immediately before (t0h) and 2 h after (t2h) supplementation of E. coli with MH medium of with isocaloric amounts of three macronutrients: BSA, D-fructose and oleic acid.