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The relative abundance of indole-3-acetonitrile (IAN) is higher in MCF-7 than in A375 cells. LC-MS chromatogram and MS spectrum of the extracellular media of (A) A375 and (B) MCF-7 cells after (t = 24 h) treatment with carbidopa and relative abundance of IAN (m/z 157.04) over 24 h. The grey area indicates the retention time of IAN (m/z 157.04, as positive ion mode M + H).
