Figure 8.

Effect of curcuminoids on CYP19A1 activity. A dose-response profile is shown indicating the inhibition of aromatase by curcuminoids. A preparation of endoplasmic reticulum obtained from JEG3 placental cells was used as a source of aromatase activity, and tritium-labeled androstenedione was used as a substrate. The CYP19A1 reaction was monitored by calculating the amount of tritiated water released by CYP19A1 during the aromatization of androstenedione. A known inhibitor of CYP19A1, anastrozole (100 nM) was used as a positive control. Curcuminoids showed a small effect on CYP19A1 activity 6.25 µg/mL and higher inhibition was observed at 12.5, 25, 50, and 100 µg/mL concentrations of curcuminoids, indicating natural curcuminoids present in C. longa are not potent inhibitors of aromatase activity. Data are presented as the mean and standard deviation of three independent replicates. One-way ANOVA was used as a statistical method to compare each sample with the control (Ctrl) giving significant results at * p < 0.05 and ** p < 0.001.