Figure 4.

Time-response of Piper genus-derived compounds on cell viability. MCF7 cells were treated with increasing concentrations of gibbilimbol B, eriopodol A, and erioquinol for 6, 12, and 24 h before 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results are expressed by setting the absorbance of the reduced MTT in the respective control (vehicle-treated) samples, i.e., absence of compounds, as 100%. The data points are representative of four independent experiments.